Can marijuana use put offspring at heightened risk for opiate addiction, even if the use stops before the offspring are conceived? Recent animal research by NIDA-supported scientists suggests that the answer may be yes.
Dr. Yasmin L. Hurd and colleagues at the Icahn School of Medicine at Mount Sinai in New York City showed that rats whose parents had been exposed as adolescents to the main psychoactive ingredient in marijuana sought heroin more vigorously than the offspring of unexposed animals. Although more research is needed to confirm and explain the findings, they are consistent with other studies suggesting that a parent’s history of drug use, even preconception, may affect a child’s brain function and behavior.
Scientists have known for a while that drugs of abuse produce some of their effects epigenetically—that is, by increasing or decreasing the rates at which the body’s genetic machinery produces certain proteins. Researchers recently reported that some epigenetic changes produced by cocaine appear to be inherited and affect the behavior of subsequent generations. In that experiment, rats whose parents had been exposed to cocaine responded differently when introduced to the drug than did rats whose parents had not been exposed.
Dr. Hurd and colleagues hypothesized that rats whose parents were exposed as adolescents to the main psychoactive ingredient in marijuana (delta-9 tetrahydrocannabinol, or THC) would inherit epigenetic changes that would alter their responses to heroin. To test the hypothesis, the researchers injected adolescent male and female rats with THC for 3 weeks on an intermittent schedule (1.5 milligram per kilogram of body weight every 3 days) that corresponds to the amounts consumed by a typical recreational marijuana user. They waited 2 to 4 weeks for the drug to wash out of the rats’ bodies, then paired and mated them.
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When the offspring of these matings reached adulthood, the researchers presented them with a lever that, when pressed, delivered heroin (30 micrograms per kilogram of body weight). At first, the animals self-administered the drug at roughly the same rates as a group of control animals whose parents had not been exposed to THC. However, when the researchers made the animals work harder for the drug—requiring them to press the active lever at least 5 times to receive a dose—those whose parents had been exposed to the drug pressed on average nearly 3 times as often as the control rats (see Figure 1).
When the researchers removed the animals’ access to heroin, the THC-exposed rats’ offspring exhibited more pronounced withdrawal symptoms, such as increased locomotion and repetitive behaviors. Also during withdrawal, the two groups of rats differed in their readiness to approach a novel stimulus in their environment.
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Using electrophysiology, the researchers also demonstrated that the offspring of the THC-exposed rats had altered neuronal functioning (see Figure 2). The specific alteration that they observed—enhanced long-term synaptic depression of medium spiny neurons in the dorsal striatum—has been associated with addiction in previous studies. The neurons are less responsive to stimulation, which inhibits an individual’s ability to adjust to experience and results in habitual and compulsive behaviors rather than adaptive ones.
To identify the epigenetic factors that might underlie the differences they had observed in the offspring of the THC-exposed animals, the researchers assayed concentrations of messenger RNA (mRNA) for key proteins in the brain. The formation of mRNA is the first step in the process of protein production, and mRNA levels indicate how much protein is being produced at a given time. The researchers’ analysis showed that, during adolescence, the THC-exposed animals’ offspring had higher levels of mRNA for glutamate receptors and for the cannabinoid 1 receptor in the ventral striatum. During adulthood, the offspring of the THC-exposed rats had less mRNA for N-methyl-D-aspartate (NMDA)-type glutamate receptors in the dorsal striatum (see Figure 3). Reduced production of glutamate receptors could underlie the reduced responsiveness to stimulation researchers observed in that brain region.
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Is It Real?
The Mount Sinai researchers took pains to rule out potential nonepigenetic explanations for the differences they observed between their groups of rats. One concern was that the THC-exposed rats’ pups might themselves be exposed to the drug during gestation, resulting in altered brain development. To preclude this possibility, the researchers postponed mating their THC-exposed animals until sensitive gas chromatography and mass spectrometry confirmed that no drug remained in the animals’ blood or brain tissue. Another concern was that the THC-exposed animals might parent differently than the unexposed animals, potentially altering their offspring’s responses to heroin. To prevent this, the researchers removed the THC-exposed animals’ pups from their parents immediately after birth and had unexposed dams raise both groups of offspring in mixed litters.
Despite these careful controls, Dr. Hurd and colleagues say that they cannot completely rule out nonepigenetic explanations for the alterations they observed in their THC-exposed rats’ offspring until they see what happens in the next two generations of their germ line. The researchers are proceeding with this work.
“The idea of cross-generational transmission of complex traits such as drug responses without alterations to the genome is contentious,” says Dr. John Satterlee, Project Officer at NIDA’s Genetics and Molecular Neurobiology Research Branch. “Is it real? And if it’s real, how is it transmitted?” he asks.
Dr. Satterlee agrees with Dr. Hurd that studies on future generations are needed to definitively rule out the possibility that nonepigenetic factors led to the observed effects in the offspring. Previous exposure to THC theoretically could affect the womb or placental formation, he says, or lead to changes in the parents’ microbiome—the assemblage of microorganisms in the gut controlling a variety of conditions and behaviors—that were then transmitted to their offspring.
“If the effect is real, it’s important,” Dr. Satterlee says. “If studies show that marijuana use also shows cross-generational effects in people, those results would add to the known dangers of the drug and amplify the importance of prevention efforts, especially those aimed at youth,” he adds.
This study was supported by NIH grants DA030359 and DA033660.
Szutorisz, H.; DiNieri, J.A.; Sweet, E. et al. Parental THC exposure leads to compulsive heroin-seeking and altered striatal synaptic plasticity in the subsequent generation. Neuropsychopharmacology. 39(6):1315-1323, 2014. Abstract
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NIDA (). Animal Study Suggests Marijuana May Affect Future Offspring’s Susceptibility to Heroin. Retrieved , from https://www.drugabuse.gov/news-events/nida-notes/2015/02/animal-study-suggests-marijuana-may-affect-future-offsprings-susceptibility-to-heroin